纤维素降解的AFM单分子识别研究
张亚男，徐胜楠，季 凡，胡玉冰纤维素降解的AFM单分子识别研究
张亚男，徐胜楠，季 凡，胡玉冰
（南京工业大学 材料科学与工程学院，江苏 南京 211816）
摘 要 提高能量转化效率一直是生物能源利用的重大难题，在单分子层面研究生物能源转化机理尤为重要。PicoTREC是一种新型生物化学探测技术，该技术可以将与AFM探针上配体相互作用的分子从复杂成分中区别出来，同时可以实时观察该分子的一系列动态过程。本文中，作者使用原子力显微镜（atomic force microscope，AFM）的PicoTREC技术，在单分子层面对纤维素酶解过程进行了实时原位观测，并分析了纤维素内切酶分子在纤维素表面的作用机理，为晶体纤维素进一步降解的机理分析奠定了基础。
关键词 纤维素；酶解；识别成像；实时；单分子
中图分类号： 文献标识码：A doi:10.3969/j.issn.1000-6281.2020.04.006
The study of cellulose biodegradation using AFM single molecular recognition technique
ZHANG Ya-nan, XU Sheng-nan, JI Fan, HU Yu-bing
（School of Materials Science and Engineering, Nanjing Tech University, NanjingJiangsu211816, China）
Abstract Improving energy conversion efficiency has always been a major project in bioenergy utilization.It is very important to study bioenergy conversion mechanism at single molecular level. The technique using both the high-resolution AFM topography image and specific recognition image is named as PicoTREC. PicoTREC is a powerful and new-type biochemical detection technology. We can distinguish one component, and then observe it in real time during a series of dynamic process. In this study, the complete enzymatic hydrolysis process of natural cellulose substrates was visualized in situ and in real time by atomic force microscopy (AFM) PicoTREC technology, and the mechanism of cellulose hydrolyzed by endonuclease was also analyzed. This work will lay a foundation for the mechanism analysis of further degradation of crystalline cellulose.
Keywords cellulose; enzymolysis; recognition imaging; real time; single molecule
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